The Protocol Exchange is an open-access resource where scientists can share their protocols, and browse those that others have added.
Exchange Protocols are accessed from a Browse page, and can be either Community or Supplier Contributed.
Most of our Exchange Protocols have been used to generate results published in primary research papers; where these papers have been indicated, they are called Associated Publications. You can quickly see this information by clicking "Show details" on your browse results. As an example, here is a complete list of Exchange Protocols associated with Nature papers.
Protocols on the Protocol Exchange are not peer-reviewed or edited, therefore the protocols highlighted are ones that are nice examples of using our format. If there are ones that you think should be included on this Top-Five list, then please let us know which are your favourites!Protocol Exchange - Five favourites.
- Neural Stem Cell Culture: Neurosphere generation, microscopical analysis and cryopreservation
- Seeing is believing: in vivo functional real-time imaging of transplanted islets using positron emission tomography (PET)
- Development of QSAR models using C-QSAR program: a regression program that has dual databases of over 21,000 QSAR models
- Probing RNA structure genome-wide using high throughput sequencing
- A method for labeling polyacrylamide gels
Exchange Protocols that have interesting commentsOne of the really nice features of both Nature Protocols and the Protocol Exchange is that it is possible to comment on protocols. While this resource is under-utilised, there are a few protocols that have very interesting discussions associated with them, and I have highlighted these below.A complete list of Exchange Protocols with comments can be found here.
- Production of neuron-preferential lentiviral vectors
- High-throughput cloning and expression in Lactococcus lactis
- Calcium flux: Indo-1 loading and sample staining procedure for simultaneous measurement of intracellular Ca2+
- Assessment of cry1Ab transgene cassette in commercial Bt corn MON810: gene, event, construct and GMO specific concurrent characterization
Labgroups that have contributed loads of protocols.
It would be really great if research laboratories used the Protocol Exchange as a way to archive and share their methods within their group, with collaborators and with the wider community.
This has not really happened yet, but there are a few people who have uploaded collections of protocols either all relating to a single research paper or, in the case of Wei Zou, to a PhD thesis.
(a series of protocols relating to a Nature Structural and Molecular Biology paper)
Jeak Ling DING"s lab (National University of Singapore) (a series of protocols relating to a Nature Immunology paper)
Labgroups that have pretty logos
When you create a labgroup, you can add an image, or logo, to go with it. There are a few really pretty ones which I have listed below.
Unfortunately the one for the Cell Migration Consortium - Denise Montell Lab (Johns Hopkins) does not seem to show nicely in storify.