Storified by · Fri, Feb 08 2013 11:44:30

@matthewherper @WillisCD @eyeslash Majority of @iontorrent users are NGS noobs with low throughput. Power users mostly buy MiSeqs.

— Daniel MacArthur · Fri, Feb 08 2013 11:44:30

@dgmacarthur @WillisCD @eyeslash I believe that power users tend to be MiSeq, but I've seen some interesting papers done on @iontorrent.

— Matthew Herper · Fri, Feb 08 2013 11:44:30

@matthewherper @WillisCD @eyeslash @iontorrent Sure. Helicos had some interesting papers too.

— Daniel MacArthur · Fri, Feb 08 2013 11:44:30

@dgmacarthur The big question: what do you hear about Proton?

— Matthew Herper · Fri, Feb 08 2013 11:44:30

@matthewherper Lower yield than a HiSeq, crappier data than a MiSeq - what’s not to like? I’m sure LIFE’s PR will do well nonetheless.

— Daniel MacArthur · Fri, Feb 08 2013 11:44:30

@dgmacarthur I'm not so sure they will. You don't buy the argument they could get cheap but good enough?

— Matthew Herper · Fri, Feb 08 2013 11:44:30

@matthewherper But they can always take SOLiD strategy: give ‘em away to inflate apparent user base. Wasn’t a winning plan, though…

— Daniel MacArthur · Fri, Feb 08 2013 11:44:30

@dgmacarthur No. What do you think of Nusbaum's arguments that bad data are ok if they're cheap?

— Matthew Herper · Fri, Feb 08 2013 11:44:30

@matthewherper @dgmacarthur comparisons presented at #PGMG12 suggested that Ion cost was actually quite similar to Illumina.

— David Mittelman · Fri, Feb 08 2013 11:44:30

@evolvability @matthewherper Raw cost, or for equivalent coverage with high-quality bases?

— Daniel MacArthur · Fri, Feb 08 2013 11:44:30

@dgmacarthur @matthewherper It was the on the last day, D. Muzny's talk, and I do not remember for sure. Do you remember? Am I mistaken?

— David Mittelman · Fri, Feb 08 2013 11:44:30

@evolvability @matthewherper My memory is very hazy - I had a grant deadline two days later IIRC. :-)

— Daniel MacArthur · Fri, Feb 08 2013 11:44:30

@dgmacarthur @matthewherper From the abstract, it says Proton generated 12GB raw seq, 92% exome targets, 20X coverage. Nothing about cost.

— David Mittelman · Fri, Feb 08 2013 11:44:30

@evolvability @matthewherper 92% at 1X and mean coverage 20X? That’s really bad - the stats are better now.

— Daniel MacArthur · Fri, Feb 08 2013 11:44:30

Storified by · Fri, Feb 08 2013 11:43:44

@matthewherper @WillisCD @eyeslash Majority of @iontorrent users are NGS noobs with low throughput. Power users mostly buy MiSeqs.

— Daniel MacArthur · Fri, Feb 08 2013 11:43:42

@dgmacarthur @WillisCD @eyeslash I believe that power users tend to be MiSeq, but I've seen some interesting papers done on @iontorrent.

— Matthew Herper · Fri, Feb 08 2013 11:43:42

@matthewherper @WillisCD @eyeslash @iontorrent Sure. Helicos had some interesting papers too.

— Daniel MacArthur · Fri, Feb 08 2013 11:43:42

@dgmacarthur The big question: what do you hear about Proton?

— Matthew Herper · Fri, Feb 08 2013 11:43:42

@matthewherper Lower yield than a HiSeq, crappier data than a MiSeq - what’s not to like? I’m sure LIFE’s PR will do well nonetheless.

— Daniel MacArthur · Fri, Feb 08 2013 11:43:42

@dgmacarthur I'm not so sure they will. You don't buy the argument they could get cheap but good enough?

— Matthew Herper · Fri, Feb 08 2013 11:43:43

@matthewherper But they can always take SOLiD strategy: give ‘em away to inflate apparent user base. Wasn’t a winning plan, though…

— Daniel MacArthur · Fri, Feb 08 2013 11:43:43

@dgmacarthur No. What do you think of Nusbaum's arguments that bad data are ok if they're cheap?

— Matthew Herper · Fri, Feb 08 2013 11:43:43

@matthewherper @dgmacarthur comparisons presented at #PGMG12 suggested that Ion cost was actually quite similar to Illumina.

— David Mittelman · Fri, Feb 08 2013 11:43:43

@evolvability @matthewherper Raw cost, or for equivalent coverage with high-quality bases?

— Daniel MacArthur · Fri, Feb 08 2013 11:43:43

@dgmacarthur @matthewherper It was the on the last day, D. Muzny's talk, and I do not remember for sure. Do you remember? Am I mistaken?

— David Mittelman · Fri, Feb 08 2013 11:43:43

@evolvability @matthewherper My memory is very hazy - I had a grant deadline two days later IIRC. :-)

— Daniel MacArthur · Fri, Feb 08 2013 11:43:44

@dgmacarthur @matthewherper From the abstract, it says Proton generated 12GB raw seq, 92% exome targets, 20X coverage. Nothing about cost.

— David Mittelman · Fri, Feb 08 2013 11:43:44

@evolvability @matthewherper 92% at 1X and mean coverage 20X? That’s really bad - the stats are better now.

— Daniel MacArthur · Fri, Feb 08 2013 11:43:44

A collection of #drunksci Tweets

Storified by · Sat, Mar 10 2012 11:17:28

+1 RT @wentrogue @mollyesque: "Drunk students solved nearly 30% more word problems than their sober peers." on.wsj.com/yMZthF #drunksci

— · Fri, Mar 09 2012 18:12:42

Champagne Science: Flute Or Coupe For The Best Bubbles? feedly.com/k/wIUo8L #drunksci

— · Fri, Mar 09 2012 18:12:42

Need a drink. #drunksci #thankgoditsfriday

— · Fri, Mar 09 2012 18:12:42

Creating diamonds from tequila? arxiv.org/abs/0806.1485 #drunksci

— · Fri, Mar 09 2012 18:12:42

Did I just read that Godzilla genome was sequenced? #drunksci

— · Fri, Mar 09 2012 18:12:42

DIY Four Loko. Because the world needed that. gizmo.do/wPHcz4 #drunksci?

— · Fri, Mar 09 2012 18:12:42

@GertyZ It also took me 5 minutes to actually type the damn thing. #drunksci #ithelpswiththestress

— · Fri, Mar 09 2012 18:12:42

Dishes done, lectures ready, dog walked & laundry folded & away. Time for gin, more gin & blogging! #DrunkSci

— · Sun, Mar 04 2012 18:14:51

More Advanced Beer and Brewing –Tips, Tricks, and Tidbits You Wish You Knew - acswebinars.org/bamforth2012 #drunksci

— · Sun, Mar 04 2012 18:14:51

"The price of being sober is being scared out of your mind." - Drive By Truckers #drunksci

— · Sun, Mar 04 2012 18:14:51

Fyi, 2nd step is defective, bad ALDH not ADH. RT @BoraZ Does beer make you blush? or, why “race” is a myth bit.ly/wiHfFY #drunksci

— · Sun, Mar 04 2012 18:14:51

RT @matthiasrascher: Quiz: The science of alcohol. bit.ly/bDpWe9 #alcohol #chemistry #drunksci

— · Sun, Mar 04 2012 18:14:51

After a week of hard lab's work, a 48h exposure to several -OH containing solutions will do the trick. #drunksci

— · Sun, Mar 04 2012 18:14:51

Friday Weird Science: Does the beer belly really exist? by @scicurious bit.ly/z3IZDZ #drunksci

— · Sun, Mar 04 2012 18:14:51

LOL. PhD thesis acknowledgment: "rest of my colleagues, for participating in quite a few “fruitful” coffee breaks." #DrunkSci

— · Sun, Mar 04 2012 18:14:51

What the Home-Brew Movement Can Teach Us About Emerging Technologies slate.com/articles/techn… via @slate #DrunkSci

— · Sun, Mar 04 2012 18:14:51

Custom ice cream rules #drunksci http://t.co/TdPrwRAf · labroides

Cerveceria alemana with a bunch of #drunksci - thx @paulhoskisson http://t.co/U9oyK1Sn · kamounlab

@DrRubidium It's not #abtk, but what about scotch? http://t.co/wV8QXNBI #drunksci? · vonoberst

#drunksci RT @kTraphagen: Oh my. Guinness jello shots w Baileys Cream topping. http://bit.ly/xGRPVx (via @neatorama )

— Kevin Zelnio · Fri, Feb 24 2012 18:55:37

Storified by · Sat, Mar 10 2012 11:15:20

NMR is down at the moment, and I have about 5 samples in need data on before continuing work #lifeinthelab #lifeintheoffice?

— · Fri, Mar 09 2012 18:13:20

My 100th blog post: wp.me/p1hrbg-j3 #lifeinthelab

— · Fri, Mar 09 2012 18:13:20

Photo of the week #lifeinthelab wp.me/p1hrbg-j3

— · Fri, Mar 09 2012 18:13:20

Why did it suddenly get freaking FREEZING in the lab? It's like they turned on the A/C. I certainly didn't do it...#magicHVAC #lifeinthelab 

— · Fri, Mar 09 2012 18:13:20

another "day in the life" post #lifeinthelab wp.me/p1hrbg-jP

— · Sun, Mar 04 2012 18:15:47

I have tried and breathed a lot of things, but dimethylformamide (DMF) vapours are among the worst. #LifeInTheLab

— · Sun, Mar 04 2012 18:15:47

New blog post: Supply and demand #lifeinthelab wp.me/p1hrbg-jn

— · Sun, Mar 04 2012 18:15:47

New post: A day in the life wp.me/p1hrbg-hF #lifeinthelab

— · Sun, Mar 04 2012 18:15:47

Photo of the week #lifeinthelab wp.me/p1hrbg-iC

— · Sun, Mar 04 2012 18:15:47

Whew. Let's hope that's the last of *that* kind of assay for a while. *massages wrist* #lifeinthelab 

— · Fri, Feb 24 2012 19:11:15

Photo of the Week #lifeinthelab wp.me/p1hrbg-gQ

— · Fri, Feb 24 2012 19:11:15

This day is sadly repetitive. #dejavu #lifeinthelab 

— · Fri, Feb 24 2012 19:11:15

Here I am at work. On a Sunday. Because this data might get us into a good journal, therefore it must be done today. #lifeinthelab 

— etoiline · Sun, Feb 19 2012 09:57:10

New blog post: The journey: clearing out an abandoned lab #lifeinthelab http://wp.me/p1hrbg-gg

— Debbie Knight · Wed, Feb 15 2012 06:01:53

Photo of the week #lifeinthelab http://wp.me/p1hrbg-gb

— Debbie Knight · Tue, Feb 14 2012 06:08:13

"@acousticgravity: Photo of the week #lifeinthelab http://wp.me/p1hrbg-fD" #seemyscience

— Dr. Watson · Mon, Feb 13 2012 12:33:16

The coolest lab clock ever http://j.mp/yWtRyc #lifeinthelab

— kurt_gielen · Fri, Feb 03 2012 12:46:08

Photobucket

RT @ajh1269: Made me smile.....successful experiment = controls worked and no one died :) #lifeinthelab

— kurt_gielen · Thu, Feb 02 2012 05:08:40

What do you feed "gallus gallus" anyway? International Laboratory Animal Technician Week!!! http://j.mp/xAE0jF #science #lifeinthelab

— kurt_gielen · Wed, Feb 01 2012 14:06:02

A place to showcase #labfrustration Tweets *note - content is currently curated by me but I hope to automate it soon

Storified by · Sat, Mar 10 2012 11:14:31

Recent encounters with #siRNA have been (unsurprisingly) rife with #labfrustration. Fingers crossed for this week

— · Sun, Mar 04 2012 18:15:47

#Threedaysgrace said it best, "I hate everything about you / Why do I love you?" #science #labfrustration #cloning

— · Fri, Feb 24 2012 19:11:50

Someone messing with the settings on my incubator, and stalling my growth... not so much #labfrustration as full-on #labrage

— · Fri, Feb 24 2012 19:11:50

Dirrrrty sequencing. Manually reading the chronograph gives me some hope. Will re-sequence tomorrow #labfrustration

— · Fri, Feb 24 2012 19:11:50

Ultimate #labfrustration this week. Fecking phage infection.

— Katie Fleming · Wed, Feb 15 2012 01:33:30

It's home time and I'm still waiting to have a meeting with the boss #labfrustration

— Jamie Doonan · Thu, Feb 02 2012 09:10:43

@cellsLV fair point, but a little full on for something that is simply meant for bonding over shared experiences. #labfrustration is #labfun

— TheLeadingEdge · Thu, Feb 02 2012 06:24:21

@nicolwatson Also love #labfrustration mine is currently genotyping!

— Michelle Campbell · Thu, Feb 02 2012 05:24:07

@elle__xx One of the best hashtags for sure! #labfrustration is also great!

— nicolwatson · Thu, Feb 02 2012 04:26:12

Tweets that are tagged with #labfrustration should have #firstworldproblems as well. http://chzb.gr/wUOKmH No offense, just cheer up!

— cellsLV · Wed, Feb 01 2012 06:37:59

The Top 10 Worst Things About Working in a Lab - Science Careers - Biotech, Pharmaceutical, Faculty, Postdoc jobs on Science Careers

Sure. That's why you have no social life. It's the lab work. Even when talking about their jobs to outsiders, your friends in other profe...

@elle__xx One of the best hashtags for sure! #labfrustration is also great!

— nicolwatson · Thu, Feb 02 2012 04:26:12

Tweets that are tagged with #labfrustration should have #firstworldproblems as well. http://chzb.gr/wUOKmH No offense, just cheer up!

— cellsLV · Wed, Feb 01 2012 06:37:59

+1 RT @leadingedgesci: @howitts_done @Darwins_cat @JeanneGarb excellent work with #seemyscience and happy to see #labfrustration in there

— Jeanne Garbarino · Wed, Feb 01 2012 05:51:04

#labfrustration I rarely do tissue culture these days, yet I'm the only one who cleans the water bath & keeps the incubator clean/humidified

— Holly · Tue, Jan 31 2012 18:10:50

#labfrustration The only other microbiologist in my year (in the lab next door, no less) is abandoning her PhD. Hate to see friends quit :(

— Holly · Tue, Jan 31 2012 14:54:07

RT @elle__xx: @nicolwatson Western buffers are a nightmare, they take the best part of a day to make #labfrustration

— TheLeadingEdge · Tue, Jan 31 2012 14:54:07

@elle__xx my #labfrustration was making up the lab's Western buffer solns is large vols and emptying cell culture aspirate - eugh!!

— Dr. Watson · Tue, Jan 31 2012 14:54:07

Also, somehow ended up with double osmolarity solution, and not sure how... #labfrustration

— Ben Mulcahy · Fri, Jan 27 2012 12:10:44

"@robjackson28: Never a good sign when your negative control has a stronger result than your positives..." Never no! #labfrustration

— Dr. Watson · Fri, Jan 27 2012 12:10:44

A curated showcase of #seemyscience Tweets

Storified by · Wed, Aug 08 2012 00:15:51

What a pinhead! This wasp has been fooled into mating with a pin coated in orchid pheremones...repeatedly #seemyscience http://pic.twitter.com/TSILEjtr · Plant Energy Biology

This makes me happy. #seemyscience http://pic.twitter.com/uYaRruTY · Jack Evans

A quick #seemyscience from earlier today - check out how bright yellow my cell pellet is. (drug I used is yellow) http://pic.twitter.com/f28bLei8 · Angela Alexander

Holy actin tails, Batman! If you are a cell, Shigella will really F U up! #seemyscience http://pic.twitter.com/23ilZLrN · Alyson S.

Came into the lab this morning to find a love note from my chemicals #seemyscience #phdchat http://pic.twitter.com/6KyHx7RZ · Noby Leong

The leaning measuring cylinder of chemistry #seemyscience #pisaeatyourheartout http://pic.twitter.com/JIt2gMtI · Noby Leong

testing stability of custom conjugate stabilizer, day 270. Good results=good day in the lab! #seemyscience pic.twitter.com/4IacIDLA · ICT

Too many crazies in the lab, so I went to play on the microscope and listen to Yanni all afternoon #seemyscience pic.twitter.com/BRcZOIgH · Christoph McDowell

This one didn't work either!!! #seemyscience pic.twitter.com/jK8GykB4 · Christoph McDowell

This photo is shit, but reminds me of Mordor for some reason #seemyscience pic.twitter.com/wBtLeM6z · Christoph McDowell

My cell culture recipe. Delicious noms for cells (sorry @johncarneyau, no cinnamon in this batch) #seemyscience http://t.co/L063WHP1 · Dr Rachael Dunlop

"@HawonK: Bought 7 minutes gel transfer device for Western blot. http://t.co/uL5lM7h8" cool benchtop device! #seemyscience · Dr. Watson

New Imaging Technology Reveals Early Changes Leading to Breast Tumours - ow.ly/9uMey #seemyscience

— · Fri, Mar 09 2012 18:13:20

Gais! Gais! I'm making cell culture medium from scratch! Look at all my ingredients! #seemyscience lockerz.com/s/190490646

— · Fri, Mar 09 2012 18:13:20

Box of goodies!! Well chemicals needed for testwork. They're goodies to me. #FIFOlife #seemyscience pic.twitter.com/3Zxm4l7v · Mags Lum

A watched column never drips #seemyscience #scienceisnotalwaysexciting pic.twitter.com/rNjd37XB · Dr Krystal

Attractive science post.ly/5nLfb magnetic beads video #seemyscience

— · Sun, Mar 04 2012 18:15:47

Why oh why will more of my OPCs not mature into Oligodendrocytes? Such pain this is causing me. #SeeMyScience pic.twitter.com/FCJieuGX · Rachel Hassan

#seemyscience Behold! The Hydra! post.ly/5mohi - Clampstand-tastic.

— · Sun, Mar 04 2012 18:15:47

#ELISA and I are matching! #seemyscience pic.twitter.com/ACaXnSAK · Lise Bollum