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International Chromosome Conference 18 - Days 2 and 3

  1. The second afternoon of the ICC had the session of epigenetic regulation of chromosome function. Since @dicentric speaking, it was left to me to tweet the talk (although I'll have an answer to the problem of tweeting during your own talk tomorrow!). Her talk was entitled "Functional centromeric epialleles on an endogenous human chromosome" but could have been shortened to simply her Twitter handle @dicentric. 


  2. Pathh1
    Human chromosome 17 has 2 adjacent satellite arrays. In 70% of cell lines one gives active centromere, 30% functionally heterozygous #icc18
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  3. Pathh1
    Beth Sullivan: functional centromeric epialleles - half of human chromosomes have structurally dicentric chromosomes @dicentric #icc18
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  4. Pathh1
    Beth Sullivan: Hsa17 also has variation in sat Higher Order Repeat size. Larger arrays seem to recruit CENPA better. #icc18
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  5. Then a cheer I'm not allowed to make until tomorrow:
  6. dicentric
    i appreciate seeing my science tweeted by someone else in my TL. thanks @Pathh1 for giving my lab's research a shout out. #icc18
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  7. Then two more epigenetic talks chaired by Bill Earnshaw
  8. dicentric
    MM: cohesion binding btw housekeeping genes & vital T-cell differentiation TCR genes. loss of cohesin impairs VDJ rearrangement. #icc18
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  9. dicentric
    Matthias Merkenschalger analyzing role of cohesin during T cell differentiation (which occurs w/o cell division!) #icc18
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  10. dicentric
    Barbara Meyer on how cohesin (chromosome glue) is loaded onto chromosomes in the nematode (microscopic worm) C. elegans. #icc18
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  11. dicentric
    Meyer: 2 forms of cohesion (REC8 vs COH3/4) are loaded and removed by two independent mechanisms. #icc18
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  12. With one more comments from @dicentric about dress-sense, before we all went off to the source of worldwide fame (? notoriety)
  13. dicentric
    @meandertail B Meyer is dressed in an awesome shiny red coat dress & black knee boots. i think she officially out-dressed me! :( #icc18
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  14. Pathh1
    #icc18 brilliant anecdotes from Malcolm Ferguson-Smith about Peter Pearson and early fluorescence work http://t.co/uGj1LU9
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  16. The third day started with our old friend and staple of every ICC, the centromere and telomere session. But lots to surprise and excite, put nicely into its context by opening lecturer Bill Earnshaw.
  17. dicentric
    BE: giving us a historical perspective of how his lab identified CREST antibodies from autoimmune sera about 25 years ago. #icc18
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  18. dicentric
    Bill Earnshaw starts off his talk w/a dedication to Heinz Hartmann Heck who did 1st Indian Muntjac karyotype. Also his wife's dad. #icc18
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  19. Pathh1
    Earnshaw started with centromere protein A, CENP-A, and is now beyond CENP-Z. Now proteomics is showing protein complexes/assemblies #icc18
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  20. dicentric
    RT @Pathh1: Earnshaw started with centromere protein A, CENP-A, and is now beyond CENP-Z. Now proteomics is showing protein complexes/assemblies #icc18
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  21. dicentric
    BE: Ska3 depletion & its effect on kinetochore & behavior of other CEN proteins. gives info on steps in kinetochore assembly #icc18
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  22. dicentric
    BE: depletion studies were done in chicken cell line DT40. this line has 100% homologous recombination, i.e. easy to knockout genes. #icc18
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  23. dicentric
    BE: super-resolution microscopy: TEEN buffer unfolds chromatin into beads-on-string. Using photoactivation localisation microscopy. #icc18
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